Structural and biochemical analysis of a novel atypically split intein reveals a conserved histidine specific to cysteine-less inteins

Protein trans-splicing mediated by a split intein reconstitutes a protein backbone from two parts. This virtually traceless autoprocessive reaction provides the basis for numerous protein engineering applications. Protein splicing typically proceeds through two thioester or oxyester intermediates in...

Descripción completa

Detalles Bibliográficos
Autores principales: Pasch, Tim, Schröder, Alexander, Kattelmann, Sabrina, Eisenstein, Miriam, Pietrokovski, Shmuel, Kümmel, Daniel, Mootz, Henning D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189870/
https://www.ncbi.nlm.nih.gov/pubmed/37206380
http://dx.doi.org/10.1039/d3sc01200j

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189870/
https://www.ncbi.nlm.nih.gov/pubmed/37206380
http://dx.doi.org/10.1039/d3sc01200j

Ejemplares similares