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Nascent MSKIK peptide cancels ribosomal stalling by arrest peptides in Escherichia coli

The insertion of the DNA sequence encoding SKIK peptide adjacent to the M start codon of a difficult-to-express protein enhances protein production in Escherichia coli. In this report, we reveal that the increased production of the SKIK-tagged protein is not due to codon usage of the SKIK sequence....

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Detalles Bibliográficos
Autores principales: Ojima-Kato, Teruyo, Nishikawa, Yuma, Furukawa, Yuki, Kojima, Takaaki, Nakano, Hideo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192921/
https://www.ncbi.nlm.nih.gov/pubmed/37028767
http://dx.doi.org/10.1016/j.jbc.2023.104676