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Nascent MSKIK peptide cancels ribosomal stalling by arrest peptides in Escherichia coli
The insertion of the DNA sequence encoding SKIK peptide adjacent to the M start codon of a difficult-to-express protein enhances protein production in Escherichia coli. In this report, we reveal that the increased production of the SKIK-tagged protein is not due to codon usage of the SKIK sequence....
Autores principales: | Ojima-Kato, Teruyo, Nishikawa, Yuma, Furukawa, Yuki, Kojima, Takaaki, Nakano, Hideo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192921/ https://www.ncbi.nlm.nih.gov/pubmed/37028767 http://dx.doi.org/10.1016/j.jbc.2023.104676 |
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