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Analysis of mannosidase I activity in interphase and mitotic cells by lectin staining and endoglycosidase H treatment
N-Glycosylation is a common protein modification catalyzed by a series of glycosylation enzymes in the endoplasmic reticulum and Golgi apparatus. Here, based on a previously established Golgi α-mannosidase-I-deficient cell line, we present a protocol to investigate the enzymatic activity of exogenou...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193293/ https://www.ncbi.nlm.nih.gov/pubmed/37148248 http://dx.doi.org/10.1016/j.xpro.2023.102283 |