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Purification and identification of oligosaccharides from Cimicifuga heracleifolia Kom. rhizomes
Even though Cimicifuga sp. is widely used in functional foods around the world, the content and structure of its oligosaccharides remain unclear. Here, we isolated a mixture of oligosaccharides from Cimicifuga heracleifolia Kom. rhizomes with a yield of 9.5% w/w. Twenty-six oligosaccharide monomers...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196342/ https://www.ncbi.nlm.nih.gov/pubmed/37215199 http://dx.doi.org/10.1016/j.fochx.2023.100706 |
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author | Cui, Liangnan Wu, Jing Wang, Xiang Yang, Xiaotong Ye, Zixin Mayo, Kevin H. Sun, Lin Zhou, Yifa |
author_facet | Cui, Liangnan Wu, Jing Wang, Xiang Yang, Xiaotong Ye, Zixin Mayo, Kevin H. Sun, Lin Zhou, Yifa |
author_sort | Cui, Liangnan |
collection | PubMed |
description | Even though Cimicifuga sp. is widely used in functional foods around the world, the content and structure of its oligosaccharides remain unclear. Here, we isolated a mixture of oligosaccharides from Cimicifuga heracleifolia Kom. rhizomes with a yield of 9.5% w/w. Twenty-six oligosaccharide monomers from the mixture were purified using optimized SEC and HILIC techniques. The oligosaccharides were identified as belonging to two groups by using HPAEC-PAD, MALDI-TOF-MS, NMR and GC–MS methylation analyses. One group belongs to sucrose and inulin type fructo-oligosaccharides (FOS) {β-d-Fruf-(2 → 1)-[β-d-Fruf-(2 [Formula: see text] 1)](n=1-12)-α-d-Glcp} with a 3–14 degree of polymerization (DP). Oligosaccharides in the other group belong to the inulo-n-ose type FOS {β-d-Fruf-(2 → 1)-[β-d-Fruf-(2 → 1)](m=0-12)-β-d-Frup} with a DP of 2–14. This appears to be the first time that these oligosaccharides have been purified from Cimicifuga heracleifolia Kom., thus providing useful information concerning the utilization of Cimicifuga heracleifolia Kom. in functional foods. |
format | Online Article Text |
id | pubmed-10196342 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-101963422023-05-20 Purification and identification of oligosaccharides from Cimicifuga heracleifolia Kom. rhizomes Cui, Liangnan Wu, Jing Wang, Xiang Yang, Xiaotong Ye, Zixin Mayo, Kevin H. Sun, Lin Zhou, Yifa Food Chem X Research Article Even though Cimicifuga sp. is widely used in functional foods around the world, the content and structure of its oligosaccharides remain unclear. Here, we isolated a mixture of oligosaccharides from Cimicifuga heracleifolia Kom. rhizomes with a yield of 9.5% w/w. Twenty-six oligosaccharide monomers from the mixture were purified using optimized SEC and HILIC techniques. The oligosaccharides were identified as belonging to two groups by using HPAEC-PAD, MALDI-TOF-MS, NMR and GC–MS methylation analyses. One group belongs to sucrose and inulin type fructo-oligosaccharides (FOS) {β-d-Fruf-(2 → 1)-[β-d-Fruf-(2 [Formula: see text] 1)](n=1-12)-α-d-Glcp} with a 3–14 degree of polymerization (DP). Oligosaccharides in the other group belong to the inulo-n-ose type FOS {β-d-Fruf-(2 → 1)-[β-d-Fruf-(2 → 1)](m=0-12)-β-d-Frup} with a DP of 2–14. This appears to be the first time that these oligosaccharides have been purified from Cimicifuga heracleifolia Kom., thus providing useful information concerning the utilization of Cimicifuga heracleifolia Kom. in functional foods. Elsevier 2023-05-06 /pmc/articles/PMC10196342/ /pubmed/37215199 http://dx.doi.org/10.1016/j.fochx.2023.100706 Text en © 2023 The Authors. Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Cui, Liangnan Wu, Jing Wang, Xiang Yang, Xiaotong Ye, Zixin Mayo, Kevin H. Sun, Lin Zhou, Yifa Purification and identification of oligosaccharides from Cimicifuga heracleifolia Kom. rhizomes |
title | Purification and identification of oligosaccharides from Cimicifuga heracleifolia Kom. rhizomes |
title_full | Purification and identification of oligosaccharides from Cimicifuga heracleifolia Kom. rhizomes |
title_fullStr | Purification and identification of oligosaccharides from Cimicifuga heracleifolia Kom. rhizomes |
title_full_unstemmed | Purification and identification of oligosaccharides from Cimicifuga heracleifolia Kom. rhizomes |
title_short | Purification and identification of oligosaccharides from Cimicifuga heracleifolia Kom. rhizomes |
title_sort | purification and identification of oligosaccharides from cimicifuga heracleifolia kom. rhizomes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196342/ https://www.ncbi.nlm.nih.gov/pubmed/37215199 http://dx.doi.org/10.1016/j.fochx.2023.100706 |
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