One-pot making of sequence-restricted DNA dumbbells

A method to build sequence-restricted DNA dumbbells was developed. 5′-exonuclease converts end sequences of DNA targets into sticky ends. Self-looping oligonucleotides with complementary 3′-overhangs are ligated to form dumbbells by DNA polymerase and ligase in a sequence-restricted manner. These re...

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Detalles Bibliográficos
Autores principales: Chang, Ya-Hui, Lin, Yu-Cheng, Liu, Hong-Hsing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196794/
https://www.ncbi.nlm.nih.gov/pubmed/37215900
http://dx.doi.org/10.1016/j.heliyon.2023.e16035

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196794/
https://www.ncbi.nlm.nih.gov/pubmed/37215900
http://dx.doi.org/10.1016/j.heliyon.2023.e16035

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