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One-pot making of sequence-restricted DNA dumbbells
A method to build sequence-restricted DNA dumbbells was developed. 5′-exonuclease converts end sequences of DNA targets into sticky ends. Self-looping oligonucleotides with complementary 3′-overhangs are ligated to form dumbbells by DNA polymerase and ligase in a sequence-restricted manner. These re...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196794/ https://www.ncbi.nlm.nih.gov/pubmed/37215900 http://dx.doi.org/10.1016/j.heliyon.2023.e16035 |