A novel enzymatic assay for the identification of 4-hydroxyphenylpyruvate dioxygenase modulators

4-hydroxyphenylpyruvate dioxygenase (HPPD) is a key enzyme involved in the pathogenesis of tyrosinemia III and cancer. Herein, we describe a spectroscopy-based assay to detect HPPD dioxygenase activity in the presence or absence of small-molecule modulators. We describe steps for transformation, exp...

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Detalles Bibliográficos
Autores principales: Parkins, Andrew, Pantouris, Georgios
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196851/
https://www.ncbi.nlm.nih.gov/pubmed/37167058
http://dx.doi.org/10.1016/j.xpro.2023.102300

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196851/
https://www.ncbi.nlm.nih.gov/pubmed/37167058
http://dx.doi.org/10.1016/j.xpro.2023.102300

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