Protocol for using single-cell sequencing to study the heterogeneity of NF1 nerve sheath tumors from clinical biospecimens

Single-cell sequencing is a powerful technology to understand the heterogeneity of clinical biospecimens. Here, we present a protocol for obtaining single-cell suspension from neurofibromatosis type 1-associated nerve sheath tumors for transcriptomic profiling on the 10x platform. We describe steps...

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Detalles Bibliográficos
Autores principales: Zhang, Xiyuan, Gopalan, Vishaka, Syed, Neeraja, Hannenhalli, Sridhar, Shern, Jack F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196853/
https://www.ncbi.nlm.nih.gov/pubmed/37167059
http://dx.doi.org/10.1016/j.xpro.2023.102297

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196853/
https://www.ncbi.nlm.nih.gov/pubmed/37167059
http://dx.doi.org/10.1016/j.xpro.2023.102297

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