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Protocol for using single-cell sequencing to study the heterogeneity of NF1 nerve sheath tumors from clinical biospecimens
Single-cell sequencing is a powerful technology to understand the heterogeneity of clinical biospecimens. Here, we present a protocol for obtaining single-cell suspension from neurofibromatosis type 1-associated nerve sheath tumors for transcriptomic profiling on the 10x platform. We describe steps...
Autores principales: | Zhang, Xiyuan, Gopalan, Vishaka, Syed, Neeraja, Hannenhalli, Sridhar, Shern, Jack F. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196853/ https://www.ncbi.nlm.nih.gov/pubmed/37167059 http://dx.doi.org/10.1016/j.xpro.2023.102297 |
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