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Influence of expression and purification protocols on Gα biochemical activity: kinetics of plant and mammalian G protein cycles

Heterotrimeric G proteins, composed of Gα, Gβ, and Gγ subunits, are a class of signal transduction complexes with broad roles in human health and agriculturally relevant plant physiological and developmental traits. In the classic paradigm, guanine nucleotide binding to the Gα subunit regulates the...

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Detalles Bibliográficos
Autores principales: Gookin, Timothy E., Chakravorty, David, Assmann, Sarah M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197700/
https://www.ncbi.nlm.nih.gov/pubmed/37214830
http://dx.doi.org/10.1101/2023.05.10.540258