CRISPRi-mediated tunable control of gene expression level with engineered single-guide RNA in Escherichia coli

Precise control of gene expression is essential for flux redistribution in metabolic pathways. Although the CRISPR interference (CRISPRi) system can effectively repress gene expression at the transcriptional level, it has still been difficult to precisely control the level without loss of specificit...

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Detalles Bibliográficos
Autores principales: Byun, Gibyuck, Yang, Jina, Seo, Sang Woo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10201414/
https://www.ncbi.nlm.nih.gov/pubmed/36999618
http://dx.doi.org/10.1093/nar/gkad234

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10201414/
https://www.ncbi.nlm.nih.gov/pubmed/36999618
http://dx.doi.org/10.1093/nar/gkad234

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