Cargando…

A new method to synthesize multiple gRNA libraries and functional mapping of mammalian H3K4me3 regions

Genetic screening based on the clustered regularly interspaced palindromic repeat (CRISPR) system has been indicated to be a powerful tool for identifying regulatory genes or cis-elements. However, when applying CRISPR screens to pinpoint functional elements at particular loci, a large number of gui...

Descripción completa

Detalles Bibliográficos
Autores principales:	Pan, Chen, Li, Ran, Shui, Liyan, Xiao, Zhengyun, Wang, Yali, Zhu, Jing, Wu, Chao, Zhang, Long, Jia, Junling, Zheng, Min
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2023
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10201432/ https://www.ncbi.nlm.nih.gov/pubmed/36938898 http://dx.doi.org/10.1093/nar/gkad198

Ejemplares similares

Uncertainty-aware and interpretable evaluation of Cas9–gRNA and Cas12a–gRNA specificity for fully matched and partially mismatched targets with Deep Kernel Learning
por: Kirillov, Bogdan, et al.
Publicado: (2021)

Generating minimum set of gRNA to cover multiple targets in multiple genomes with MINORg
por: Lee, Rachelle R Q, et al.
Publicado: (2023)

Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease
por: Fujii, Wataru, et al.
Publicado: (2013)

SMAP design: a multiplex PCR amplicon and gRNA design tool to screen for natural and CRISPR-induced genetic variation
por: Develtere, Ward, et al.
Publicado: (2023)

A platform for functional assessment of large variant libraries in mammalian cells
por: Matreyek, Kenneth A., et al.
Publicado: (2017)

An improved platform for functional assessment of large protein libraries in mammalian cells
por: Matreyek, Kenneth A, et al.
Publicado: (2020)

A multipurpose vector system for the screening of libraries in bacteria, insect and mammalian cells and expression in vivo
por: Laitinen, Olli H., et al.
Publicado: (2005)

Illumina-based RiboMethSeq approach for mapping of 2′-O-Me residues in RNA
por: Marchand, Virginie, et al.
Publicado: (2016)

Development and application of a recombination-based library versus library high- throughput yeast two-hybrid (RLL-Y2H) screening system
por: Yang, Fang, et al.
Publicado: (2018)

CpG_MI: a novel approach for identifying functional CpG islands in mammalian genomes
por: Su, Jianzhong, et al.
Publicado: (2010)

Direct-seq: programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen
por: Song, Qingkai, et al.
Publicado: (2020)

Error correction of microchip synthesized genes using Surveyor nuclease
por: Saaem, Ishtiaq, et al.
Publicado: (2012)

Error removal in microchip-synthesized DNA using immobilized MutS
por: Wan, Wen, et al.
Publicado: (2014)

An engineered mammalian band-pass network
por: Greber, David, et al.
Publicado: (2010)

Targeting individual cells by barcode in pooled sequence libraries
por: Ranu, Navpreet, et al.
Publicado: (2019)

SyMAP v3.4: a turnkey synteny system with application to plant genomes
por: Soderlund, Carol, et al.
Publicado: (2011)

Mutagenesis of diploid mammalian genes by gene entrapment
por: Lin, Qing, et al.
Publicado: (2006)

Transformation of isolated mammalian mitochondria by bacterial conjugation
por: Yoon, Young Geol, et al.
Publicado: (2005)

Targeted ‘knockdown’ of spliceosome function in mammalian cells
por: Matter, Nathalie, et al.
Publicado: (2005)

Bacteriophage Mu integration in yeast and mammalian genomes
por: Paatero, Anja O., et al.
Publicado: (2008)

Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging
por: Hong, Yu, et al.
Publicado: (2018)

The use of multiple displacement amplification to amplify complex DNA libraries
por: Fullwood, Melissa J., et al.
Publicado: (2008)

Selection of functional human antibodies from retroviral display libraries
por: Urban, Johannes H., et al.
Publicado: (2005)

Integrase-directed recovery of functional genes from genomic libraries
por: Rowe-Magnus, Dean A.
Publicado: (2009)

A transposase strategy for creating libraries of circularly permuted proteins
por: Mehta, Manan M., et al.
Publicado: (2012)

Phage display peptide libraries: deviations from randomness and correctives
por: Ryvkin, Arie, et al.
Publicado: (2018)

Flexible CRISPR library construction using parallel oligonucleotide retrieval
por: Read, Abigail, et al.
Publicado: (2017)

Extensive libraries of gene truncation variants generated by in vitro transposition
por: Morelli, Aleardo, et al.
Publicado: (2017)

Targeted insertional mutagenesis libraries for deep domain insertion profiling
por: Coyote-Maestas, Willow, et al.
Publicado: (2020)

Short-hairpin RNAs synthesized by T7 phage polymerase do not induce interferon
por: Gondai, Takuma, et al.
Publicado: (2008)

MeShClust: an intelligent tool for clustering DNA sequences
por: James, Benjamin T, et al.
Publicado: (2018)

Genome-wide mapping of G-quadruplex structures with CUT&Tag
por: Lyu, Jing, et al.
Publicado: (2021)

A complementation method for functional analysis of mammalian genes
por: Gonzalez-Santos, Juana Maria, et al.
Publicado: (2005)

Vitamin H-regulated transgene expression in mammalian cells
por: Weber, Wilfried, et al.
Publicado: (2007)

A synthetic mammalian electro-genetic transcription circuit
por: Weber, Wilfried, et al.
Publicado: (2009)

Toxin–antitoxin based transgene expression in mammalian cells
por: Nehlsen, K., et al.
Publicado: (2010)

Multi-chromatic control of mammalian gene expression and signaling
por: Müller, Konrad, et al.
Publicado: (2013)

Synthetic mammalian trigger-controlled bipartite transcription factors
por: Folcher, Marc, et al.
Publicado: (2013)

Promoter library designed for fine-tuned gene expression in Pichia pastoris
por: Hartner, Franz S., et al.
Publicado: (2008)

Covalent antibody display—an in vitro antibody-DNA library selection system
por: Reiersen, Herald, et al.
Publicado: (2005)

Cannot write session to /tmp/vufind_sessions/sess_om441r0ge97igf7e5iogtrat8s