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A preliminary study of the use of MinION sequencing to specifically detect Shiga toxin-producing Escherichia coli in culture swipes containing multiple serovars of this species

An important challenge relating to clinical diagnostics of the foodborne pathogen Shiga toxin-producing E. coli (STEC), is that PCR-detection of the shiga-toxin gene (stx) in DNA from stool samples can be accompanied by a failure to identify an STEC isolate in pure culture on agar. In this study, we...

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Detalles Bibliográficos
Autores principales: Tunsjø, Hege S., Ullmann, Ingvild Falkum, Charnock, Colin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10202931/
https://www.ncbi.nlm.nih.gov/pubmed/37217775
http://dx.doi.org/10.1038/s41598-023-35279-1