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Response Surface Methodology to Optimize the Expression Efficiency of Recombinant Reteplase
BACKGROUND: Over expression of Reteplase enzyme has already been studies in the periplasmic space of Escherichia coli (E. coli). However, the role different factors in its expresssin rate remained to be elucidated. OBJECTIVES: Optical cell density (OD), IPTG concentration, and expression time are hi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Institute of Genetic Engineering and Biotechnology
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10203180/ https://www.ncbi.nlm.nih.gov/pubmed/37228628 http://dx.doi.org/10.30498/ijb.2023.330285.3288 |