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Response Surface Methodology to Optimize the Expression Efficiency of Recombinant Reteplase

BACKGROUND: Over expression of Reteplase enzyme has already been studies in the periplasmic space of Escherichia coli (E. coli). However, the role different factors in its expresssin rate remained to be elucidated. OBJECTIVES: Optical cell density (OD), IPTG concentration, and expression time are hi...

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Detalles Bibliográficos
Autores principales:	Farzaneh, Farhad, Mirzaie, Sako, Dehnavi, Ehsan, Aghaeepoor, Mojtaba, Farzaneh, Shirin, Pourzardosht, Navid, Khalili, Saeed
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	National Institute of Genetic Engineering and Biotechnology 2023
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10203180/ https://www.ncbi.nlm.nih.gov/pubmed/37228628 http://dx.doi.org/10.30498/ijb.2023.330285.3288

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