The effects of nature‐inspired amino acid substitutions on structural and biochemical properties of the E. coli L‐asparaginase EcAIII

The Escherichia coli enzyme EcAIII catalyzes the hydrolysis of L‐Asn to L‐Asp and ammonia. Using a nature‐inspired mutagenesis approach, we designed and produced five new EcAIII variants (M200I, M200L, M200K, M200T, M200W). The modified proteins were characterized by spectroscopic and crystallograph...

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Detalles Bibliográficos
Autores principales: Janicki, Maciej, Ściuk, Anna, Zielezinski, Andrzej, Ruszkowski, Milosz, Ludwików, Agnieszka, Karlowski, Wojciech M., Jaskolski, Mariusz, Loch, Joanna I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2023
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204187/
https://www.ncbi.nlm.nih.gov/pubmed/37095066
http://dx.doi.org/10.1002/pro.4647

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204187/
https://www.ncbi.nlm.nih.gov/pubmed/37095066
http://dx.doi.org/10.1002/pro.4647

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