Efficient Purification of Polyhistidine-Tagged Recombinant Proteins Using Functionalized Corundum Particles

Immobilized metal affinity chromatography (IMAC) is a popular and valuable method for the affinity purification of polyhistidine-tagged recombinant proteins. However, it often shows practical limitations, which might require cumbersome optimizations, additional polishing, and enrichment steps. Here,...

Descripción completa

Detalles Bibliográficos
Autores principales: Völzke, Jule L., Smatty, Sarah, Döring, Sarah, Ewald, Shireen, Oelze, Marcus, Fratzke, Franziska, Flemig, Sabine, Konthur, Zoltán, Weller, Michael G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204482/
https://www.ncbi.nlm.nih.gov/pubmed/37218748
http://dx.doi.org/10.3390/biotech12020031

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204482/
https://www.ncbi.nlm.nih.gov/pubmed/37218748
http://dx.doi.org/10.3390/biotech12020031

Ejemplares similares