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The type IIS restriction enzyme MmeI can cut across a double-strand break

BACKGROUND: Type-IIS restriction enzymes cut outside their recognition sites, allowing them to remove their binding sites upon digestion. This feature has resulted in their wide application in molecular biology techniques, including seamless cloning methods, enzymatic CRISPR library generation, and...

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Detalles Bibliográficos
Autores principales: Tasnim, Maliha, Selph, T. Jacob, Olcott, Jason, Hill, Jonathon T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10209223/
https://www.ncbi.nlm.nih.gov/pubmed/37031321
http://dx.doi.org/10.1007/s11033-023-08375-8