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Quantitation of nucleoprotein complexes by UV absorbance and Bradford assay

Despite the importance of studying nucleoprotein complexes, no appropriate method for quantifying them is available. Here, a UV absorbance method using the formula “C(mg/mL) = 1.55A(280) – 0.76A(260)” were applied to quantify nucleoprotein complexes. After modification using two paired A(260) and A(...

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Detalles Bibliográficos
Autores principales: Chen, Jiang, Luo, Hao, Tao, Mei, Liu, Zhongchuan, Wang, Ganggang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Biophysics Reports Editorial Office 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10210059/
https://www.ncbi.nlm.nih.gov/pubmed/37288369
http://dx.doi.org/10.52601/bpr.2021.210028