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Analysis of the ARTIC V4 and V4.1 SARS-CoV-2 primers and their impact on the detection of Omicron BA.1 and BA.2 lineage-defining mutations

The ARTIC protocol uses a multiplexed PCR approach with two primer pools tiling the entire SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) genome. Primer pool updates are necessary for accurate amplicon sequencing of evolving SARS-CoV-2 variants with novel mutations. The suitability of...

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Detalles Bibliográficos
Autores principales: Ulhuq, Fatima R., Barge, Madhuri, Falconer, Kerry, Wild, Jonathan, Fernandes, Goncalo, Gallagher, Abbie, McGinley, Suzie, Sugadol, Ahmad, Tariq, Muhammad, Maloney, Daniel, Kenicer, Juliet, Dewar, Rebecca, Templeton, Kate, McHugh, Martin P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10210939/
https://www.ncbi.nlm.nih.gov/pubmed/37083576
http://dx.doi.org/10.1099/mgen.0.000991