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Measuring in vitro ATPase Activity with High Sensitivity Using Radiolabeled ATP
ATPase assays are a common tool for the characterization of purified ATPases. Here, we describe a radioactive [γ-(32)P]-ATP-based approach, utilizing complex formation with molybdate for phase separation of the free phosphate from non-hydrolyzed, intact ATP. The high sensitivity of this assay, compa...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Bio-Protocol
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10213075/ https://www.ncbi.nlm.nih.gov/pubmed/37251095 http://dx.doi.org/10.21769/BioProtoc.4676 |