A quadruplex real-time PCR assay combined with a conventional PCR for the differential detection of Marek’s disease virus vaccines and field strains

To evaluate the effect of the vaccine and differentiate vaccine from virulent MDV, a new quadruplex real-time PCR assay based on TaqMan probes was developed to differentiate and accurately quantify HVT, CVI988 and virulent MDV-1. The results showed that the limit of detection (LOD) of the new assay...

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Detalles Bibliográficos
Autores principales: Wu, Shaopeng, Ding, Tian, Shao, Hongxia, Qian, Kun, Ye, Jianqiang, Qin, Aijian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Veterinary Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10213282/
https://www.ncbi.nlm.nih.gov/pubmed/37252401
http://dx.doi.org/10.3389/fvets.2023.1161441

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10213282/
https://www.ncbi.nlm.nih.gov/pubmed/37252401
http://dx.doi.org/10.3389/fvets.2023.1161441

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