Validation of an HPLC-CAD Method for Determination of Lipid Content in LNP-Encapsulated COVID-19 mRNA Vaccines

Lipid nanoparticles (LNPs) are widely used as delivery systems for mRNA vaccines. The stability and bilayer fluidity of LNPs are determined by the properties and contents of the various lipids used in the formulation system, and the delivery efficiency of LNPs largely depends on the lipid compositio...

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Autores principales: Yu, Xiaojuan, Yu, Chuanfei, Wu, Xiaohong, Cui, Yu, Liu, Xiaoda, Jin, Yan, Li, Yuhua, Wang, Lan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222414/
https://www.ncbi.nlm.nih.gov/pubmed/37243041
http://dx.doi.org/10.3390/vaccines11050937
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author Yu, Xiaojuan
Yu, Chuanfei
Wu, Xiaohong
Cui, Yu
Liu, Xiaoda
Jin, Yan
Li, Yuhua
Wang, Lan
author_facet Yu, Xiaojuan
Yu, Chuanfei
Wu, Xiaohong
Cui, Yu
Liu, Xiaoda
Jin, Yan
Li, Yuhua
Wang, Lan
author_sort Yu, Xiaojuan
collection PubMed
description Lipid nanoparticles (LNPs) are widely used as delivery systems for mRNA vaccines. The stability and bilayer fluidity of LNPs are determined by the properties and contents of the various lipids used in the formulation system, and the delivery efficiency of LNPs largely depends on the lipid composition. For the quality control of such vaccines, here we developed and validated an HPLC-CAD method to identify and determine the contents of four lipids in an LNP-encapsulated COVID-19 mRNA vaccine to support lipid analysis for the development of new drugs and vaccines.
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spelling pubmed-102224142023-05-28 Validation of an HPLC-CAD Method for Determination of Lipid Content in LNP-Encapsulated COVID-19 mRNA Vaccines Yu, Xiaojuan Yu, Chuanfei Wu, Xiaohong Cui, Yu Liu, Xiaoda Jin, Yan Li, Yuhua Wang, Lan Vaccines (Basel) Article Lipid nanoparticles (LNPs) are widely used as delivery systems for mRNA vaccines. The stability and bilayer fluidity of LNPs are determined by the properties and contents of the various lipids used in the formulation system, and the delivery efficiency of LNPs largely depends on the lipid composition. For the quality control of such vaccines, here we developed and validated an HPLC-CAD method to identify and determine the contents of four lipids in an LNP-encapsulated COVID-19 mRNA vaccine to support lipid analysis for the development of new drugs and vaccines. MDPI 2023-05-04 /pmc/articles/PMC10222414/ /pubmed/37243041 http://dx.doi.org/10.3390/vaccines11050937 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yu, Xiaojuan
Yu, Chuanfei
Wu, Xiaohong
Cui, Yu
Liu, Xiaoda
Jin, Yan
Li, Yuhua
Wang, Lan
Validation of an HPLC-CAD Method for Determination of Lipid Content in LNP-Encapsulated COVID-19 mRNA Vaccines
title Validation of an HPLC-CAD Method for Determination of Lipid Content in LNP-Encapsulated COVID-19 mRNA Vaccines
title_full Validation of an HPLC-CAD Method for Determination of Lipid Content in LNP-Encapsulated COVID-19 mRNA Vaccines
title_fullStr Validation of an HPLC-CAD Method for Determination of Lipid Content in LNP-Encapsulated COVID-19 mRNA Vaccines
title_full_unstemmed Validation of an HPLC-CAD Method for Determination of Lipid Content in LNP-Encapsulated COVID-19 mRNA Vaccines
title_short Validation of an HPLC-CAD Method for Determination of Lipid Content in LNP-Encapsulated COVID-19 mRNA Vaccines
title_sort validation of an hplc-cad method for determination of lipid content in lnp-encapsulated covid-19 mrna vaccines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222414/
https://www.ncbi.nlm.nih.gov/pubmed/37243041
http://dx.doi.org/10.3390/vaccines11050937
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