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Investigations of Ascorbic Acid Synthesis and Distribution in Broiler Tissues at Different Post-Hatch Days

Ascorbic acid (AA) is an indispensable nutrient required to sustain optimal poultry health and performance, which is commonly excluded from the diet of broilers. To investigate the synthesis and distribution of AA during broiler growth and clarify its possible turnover, 144 1 d old healthy Arbor Acr...

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Autores principales: Gan, Liping, Zhao, Yifeng, Wang, Peng, Zhao, Chenkai, Li, Yilei, Huang, Weihao, Shi, Liuying, Cui, Yaoming, Qiao, Hanzhen, Wang, Jinrong, Guo, Yuming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222958/
https://www.ncbi.nlm.nih.gov/pubmed/37240782
http://dx.doi.org/10.3390/life13051137
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author Gan, Liping
Zhao, Yifeng
Wang, Peng
Zhao, Chenkai
Li, Yilei
Huang, Weihao
Shi, Liuying
Cui, Yaoming
Qiao, Hanzhen
Wang, Jinrong
Guo, Yuming
author_facet Gan, Liping
Zhao, Yifeng
Wang, Peng
Zhao, Chenkai
Li, Yilei
Huang, Weihao
Shi, Liuying
Cui, Yaoming
Qiao, Hanzhen
Wang, Jinrong
Guo, Yuming
author_sort Gan, Liping
collection PubMed
description Ascorbic acid (AA) is an indispensable nutrient required to sustain optimal poultry health and performance, which is commonly excluded from the diet of broilers. To investigate the synthesis and distribution of AA during broiler growth and clarify its possible turnover, 144 1 d old healthy Arbor Acres broilers with a body weight of approximately 41 g were randomly assigned to eight groups of 18 broilers each. The kidney, liver, ileum, and spleen of one bird from each group were collected every week until 42 d to detect the synthesis capacity, tissue distribution, and transporter gene expression of AA. The results showed that kidney L-gulonolactone oxidase (GLO) activity responded quadratically (p < 0.001), with maximum activity observed at 7 to 21 d old. Hepatic total AA and dehydroascrobate (DHA) concentration increased linearly (p < 0.001) with age, as did splenic total AA (p < 0.001). In the ileum, mRNA expression of sodium vitamin C transporter 1/2 (SVCT1/2) decreased with the growing age of the broilers (p < 0.05). The expression of SVCT1 in the kidney was not influenced by the growing age of the broilers. The progressive buildup of AA in the liver and spleen of broilers as they age implies an amplified demand for this nutrient. The waning synthesis capacity over time, however, raises concerns regarding the possible inadequacy of AA in the latter growth phase of broilers. The addition of AA to the broilers’ diet might have the potential to optimize their performance. However, the effectiveness of such dietary supplementation requires further investigation.
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spelling pubmed-102229582023-05-28 Investigations of Ascorbic Acid Synthesis and Distribution in Broiler Tissues at Different Post-Hatch Days Gan, Liping Zhao, Yifeng Wang, Peng Zhao, Chenkai Li, Yilei Huang, Weihao Shi, Liuying Cui, Yaoming Qiao, Hanzhen Wang, Jinrong Guo, Yuming Life (Basel) Article Ascorbic acid (AA) is an indispensable nutrient required to sustain optimal poultry health and performance, which is commonly excluded from the diet of broilers. To investigate the synthesis and distribution of AA during broiler growth and clarify its possible turnover, 144 1 d old healthy Arbor Acres broilers with a body weight of approximately 41 g were randomly assigned to eight groups of 18 broilers each. The kidney, liver, ileum, and spleen of one bird from each group were collected every week until 42 d to detect the synthesis capacity, tissue distribution, and transporter gene expression of AA. The results showed that kidney L-gulonolactone oxidase (GLO) activity responded quadratically (p < 0.001), with maximum activity observed at 7 to 21 d old. Hepatic total AA and dehydroascrobate (DHA) concentration increased linearly (p < 0.001) with age, as did splenic total AA (p < 0.001). In the ileum, mRNA expression of sodium vitamin C transporter 1/2 (SVCT1/2) decreased with the growing age of the broilers (p < 0.05). The expression of SVCT1 in the kidney was not influenced by the growing age of the broilers. The progressive buildup of AA in the liver and spleen of broilers as they age implies an amplified demand for this nutrient. The waning synthesis capacity over time, however, raises concerns regarding the possible inadequacy of AA in the latter growth phase of broilers. The addition of AA to the broilers’ diet might have the potential to optimize their performance. However, the effectiveness of such dietary supplementation requires further investigation. MDPI 2023-05-06 /pmc/articles/PMC10222958/ /pubmed/37240782 http://dx.doi.org/10.3390/life13051137 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gan, Liping
Zhao, Yifeng
Wang, Peng
Zhao, Chenkai
Li, Yilei
Huang, Weihao
Shi, Liuying
Cui, Yaoming
Qiao, Hanzhen
Wang, Jinrong
Guo, Yuming
Investigations of Ascorbic Acid Synthesis and Distribution in Broiler Tissues at Different Post-Hatch Days
title Investigations of Ascorbic Acid Synthesis and Distribution in Broiler Tissues at Different Post-Hatch Days
title_full Investigations of Ascorbic Acid Synthesis and Distribution in Broiler Tissues at Different Post-Hatch Days
title_fullStr Investigations of Ascorbic Acid Synthesis and Distribution in Broiler Tissues at Different Post-Hatch Days
title_full_unstemmed Investigations of Ascorbic Acid Synthesis and Distribution in Broiler Tissues at Different Post-Hatch Days
title_short Investigations of Ascorbic Acid Synthesis and Distribution in Broiler Tissues at Different Post-Hatch Days
title_sort investigations of ascorbic acid synthesis and distribution in broiler tissues at different post-hatch days
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222958/
https://www.ncbi.nlm.nih.gov/pubmed/37240782
http://dx.doi.org/10.3390/life13051137
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