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Development of a Crystal Digital RT-PCR for the Detection of Atypical Porcine Pestivirus

SIMPLE SUMMARY: Atypical porcine pestivirus (APPV), a newly discovered virus, is associated with the type A-II congenital tremor (CT) in neonatal piglets. Here, the specific primers and probe were designed, the reaction conditions were optimized, and a crystal digital RT-PCR (cdRT-PCR) and real-time...

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Detalles Bibliográficos
Autores principales: Liu, Huixin, Shi, Kaichuang, Feng, Shuping, Yin, Yanwen, Long, Feng, Si, Hongbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223196/
https://www.ncbi.nlm.nih.gov/pubmed/37235413
http://dx.doi.org/10.3390/vetsci10050330