Exploitation of a Type 1 Toxin–Antitoxin System as an Inducible Counter-Selective Marker for Genome Editing in the Acetogen Eubacterium limosum

Targeted mutations in the anaerobic methylotroph Eubacterium limosum have previously been obtained using CRISPR-based mutagenesis methods. In this study, a RelB-family toxin from Eubacterium callanderi was placed under the control of an anhydrotetracycline-sensitive promoter, forming an inducible co...

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Detalles Bibliográficos
Autores principales: Millard, James, Agius, Alexander, Zhang, Ying, Soucaille, Philippe, Minton, Nigel Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223405/
https://www.ncbi.nlm.nih.gov/pubmed/37317230
http://dx.doi.org/10.3390/microorganisms11051256

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223405/
https://www.ncbi.nlm.nih.gov/pubmed/37317230
http://dx.doi.org/10.3390/microorganisms11051256

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