Cargando…

The Potent Antitumor Activity of Smp43 against Non-Small-Cell Lung Cancer A549 Cells via Inducing Membranolysis and Mitochondrial Dysfunction

Research has been conducted to investigate the potential application of scorpion venom-derived peptides in cancer therapy. Smp43, a cationic antimicrobial peptide from Scorpio maurus palmatus venom, has been found to exhibit suppressive activity against the proliferation of multiple cancer cell line...

Descripción completa

Detalles Bibliográficos
Autores principales: Deng, Ze, Gao, Yahua, Nguyen, Tienthanh, Chai, Jinwei, Wu, Jiena, Li, Jiali, Abdel-Rahman, Mohamed A., Xu, Xueqing, Chen, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223799/
https://www.ncbi.nlm.nih.gov/pubmed/37235381
http://dx.doi.org/10.3390/toxins15050347
_version_ 1785050028007489536
author Deng, Ze
Gao, Yahua
Nguyen, Tienthanh
Chai, Jinwei
Wu, Jiena
Li, Jiali
Abdel-Rahman, Mohamed A.
Xu, Xueqing
Chen, Xin
author_facet Deng, Ze
Gao, Yahua
Nguyen, Tienthanh
Chai, Jinwei
Wu, Jiena
Li, Jiali
Abdel-Rahman, Mohamed A.
Xu, Xueqing
Chen, Xin
author_sort Deng, Ze
collection PubMed
description Research has been conducted to investigate the potential application of scorpion venom-derived peptides in cancer therapy. Smp43, a cationic antimicrobial peptide from Scorpio maurus palmatus venom, has been found to exhibit suppressive activity against the proliferation of multiple cancer cell lines. However, its impact on non-small-cell lung cancer (NSCLC) cell lines has not been previously investigated. This study aimed to determine the cytotoxicity of Smp43 towards various NSCLC cell lines, particularly A549 cells with an IC(50) value of 2.58 μM. The results indicated that Smp43 was internalized into A549 cells through membranolysis and endocytosis, which caused cytoskeleton disorganization, a loss of mitochondrial membrane potential, an accumulation of reactive oxygen species (ROS), and abnormal apoptosis, cell cycle distribution, and autophagy due to mitochondrial dysfunction. Additionally, the study explored the in vivo protective effect of Smp43 in xenograft mice. The findings suggest that Smp43 has potential anticarcinoma properties exerted via the inducement of cellular processes related to cell membrane disruption and mitochondrial dysfunction.
format Online
Article
Text
id pubmed-10223799
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102237992023-05-28 The Potent Antitumor Activity of Smp43 against Non-Small-Cell Lung Cancer A549 Cells via Inducing Membranolysis and Mitochondrial Dysfunction Deng, Ze Gao, Yahua Nguyen, Tienthanh Chai, Jinwei Wu, Jiena Li, Jiali Abdel-Rahman, Mohamed A. Xu, Xueqing Chen, Xin Toxins (Basel) Article Research has been conducted to investigate the potential application of scorpion venom-derived peptides in cancer therapy. Smp43, a cationic antimicrobial peptide from Scorpio maurus palmatus venom, has been found to exhibit suppressive activity against the proliferation of multiple cancer cell lines. However, its impact on non-small-cell lung cancer (NSCLC) cell lines has not been previously investigated. This study aimed to determine the cytotoxicity of Smp43 towards various NSCLC cell lines, particularly A549 cells with an IC(50) value of 2.58 μM. The results indicated that Smp43 was internalized into A549 cells through membranolysis and endocytosis, which caused cytoskeleton disorganization, a loss of mitochondrial membrane potential, an accumulation of reactive oxygen species (ROS), and abnormal apoptosis, cell cycle distribution, and autophagy due to mitochondrial dysfunction. Additionally, the study explored the in vivo protective effect of Smp43 in xenograft mice. The findings suggest that Smp43 has potential anticarcinoma properties exerted via the inducement of cellular processes related to cell membrane disruption and mitochondrial dysfunction. MDPI 2023-05-19 /pmc/articles/PMC10223799/ /pubmed/37235381 http://dx.doi.org/10.3390/toxins15050347 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Deng, Ze
Gao, Yahua
Nguyen, Tienthanh
Chai, Jinwei
Wu, Jiena
Li, Jiali
Abdel-Rahman, Mohamed A.
Xu, Xueqing
Chen, Xin
The Potent Antitumor Activity of Smp43 against Non-Small-Cell Lung Cancer A549 Cells via Inducing Membranolysis and Mitochondrial Dysfunction
title The Potent Antitumor Activity of Smp43 against Non-Small-Cell Lung Cancer A549 Cells via Inducing Membranolysis and Mitochondrial Dysfunction
title_full The Potent Antitumor Activity of Smp43 against Non-Small-Cell Lung Cancer A549 Cells via Inducing Membranolysis and Mitochondrial Dysfunction
title_fullStr The Potent Antitumor Activity of Smp43 against Non-Small-Cell Lung Cancer A549 Cells via Inducing Membranolysis and Mitochondrial Dysfunction
title_full_unstemmed The Potent Antitumor Activity of Smp43 against Non-Small-Cell Lung Cancer A549 Cells via Inducing Membranolysis and Mitochondrial Dysfunction
title_short The Potent Antitumor Activity of Smp43 against Non-Small-Cell Lung Cancer A549 Cells via Inducing Membranolysis and Mitochondrial Dysfunction
title_sort potent antitumor activity of smp43 against non-small-cell lung cancer a549 cells via inducing membranolysis and mitochondrial dysfunction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10223799/
https://www.ncbi.nlm.nih.gov/pubmed/37235381
http://dx.doi.org/10.3390/toxins15050347
work_keys_str_mv AT dengze thepotentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction
AT gaoyahua thepotentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction
AT nguyentienthanh thepotentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction
AT chaijinwei thepotentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction
AT wujiena thepotentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction
AT lijiali thepotentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction
AT abdelrahmanmohameda thepotentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction
AT xuxueqing thepotentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction
AT chenxin thepotentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction
AT dengze potentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction
AT gaoyahua potentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction
AT nguyentienthanh potentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction
AT chaijinwei potentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction
AT wujiena potentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction
AT lijiali potentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction
AT abdelrahmanmohameda potentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction
AT xuxueqing potentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction
AT chenxin potentantitumoractivityofsmp43againstnonsmallcelllungcancera549cellsviainducingmembranolysisandmitochondrialdysfunction