Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil

The main objective of this study was to investigate the dynamic of SARS-CoV-2 viral excretion in rectal swab (RS), saliva, and nasopharyngeal swab (NS) samples from symptomatic patients and asymptomatic contacts. In addition, in order to evaluate the replication potential of SARS-CoV-2 in the gastro...

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Autores principales: Ribeiro, Ieda Pereira, do Nascimento, Lilian Gonçalves, Tort, Luis Fernando Lopez, Pereira, Elisa Cavalcante, Menezes, Lidiane Souza Raphael, Malta, Fabio Correia, de Oliveira, Barbara Cristina Euzebio Pereira Dias, Rodrigues, João Paulo, Manso, Pedro Paulo de Abreu, Pelajo, Marcelo, Bonaldo, Myrna Cristina, Silva, Paola Cristina Resende, Siqueira, Marilda Mendonça, Brasil, Patricia, Fumian, Tulio Machado
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224316/
https://www.ncbi.nlm.nih.gov/pubmed/37243238
http://dx.doi.org/10.3390/v15051152
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author Ribeiro, Ieda Pereira
do Nascimento, Lilian Gonçalves
Tort, Luis Fernando Lopez
Pereira, Elisa Cavalcante
Menezes, Lidiane Souza Raphael
Malta, Fabio Correia
de Oliveira, Barbara Cristina Euzebio Pereira Dias
Rodrigues, João Paulo
Manso, Pedro Paulo de Abreu
Pelajo, Marcelo
Bonaldo, Myrna Cristina
Silva, Paola Cristina Resende
Siqueira, Marilda Mendonça
Brasil, Patricia
Fumian, Tulio Machado
author_facet Ribeiro, Ieda Pereira
do Nascimento, Lilian Gonçalves
Tort, Luis Fernando Lopez
Pereira, Elisa Cavalcante
Menezes, Lidiane Souza Raphael
Malta, Fabio Correia
de Oliveira, Barbara Cristina Euzebio Pereira Dias
Rodrigues, João Paulo
Manso, Pedro Paulo de Abreu
Pelajo, Marcelo
Bonaldo, Myrna Cristina
Silva, Paola Cristina Resende
Siqueira, Marilda Mendonça
Brasil, Patricia
Fumian, Tulio Machado
author_sort Ribeiro, Ieda Pereira
collection PubMed
description The main objective of this study was to investigate the dynamic of SARS-CoV-2 viral excretion in rectal swab (RS), saliva, and nasopharyngeal swab (NS) samples from symptomatic patients and asymptomatic contacts. In addition, in order to evaluate the replication potential of SARS-CoV-2 in the gastrointestinal (GI) tract and the excretion of infectious SARS-CoV-2 from feces, we investigated the presence of subgenomic nucleoprotein gene (N) mRNA (sgN) in RS samples and cytopathic effects in Vero cell culture. A prospective cohort study was performed to collect samples from symptomatic patients and contacts in Rio de Janeiro, Brazil, from May to October 2020. One hundred and seventy-six patients had samples collected at home visits and/or during the follow up, resulting in a total of 1633 RS, saliva, or NS samples. SARS-CoV-2 RNA was detected in 130 (73.9%) patients who had at least one sample that tested positive for SARS-CoV-2. The presence of replicating SARS-CoV-2 in RS samples, measured by the detection of sgN mRNA, was successfully achieved in 19.4% (6/31) of samples, whilst infectious SARS-CoV-2, measured by the generation of cytopathic effects in cell culture, was identified in only one RS sample. Although rare, our results demonstrated the replication capacity of SARS-CoV-2 in the GI tract, and infectious viruses in one RS sample. There is still a gap in the knowledge regarding SARS-CoV-2 fecal–oral transmission. Additional studies are warranted to investigate fecal or wastewater exposure as a risk factor for transmission in human populations.
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spelling pubmed-102243162023-05-28 Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil Ribeiro, Ieda Pereira do Nascimento, Lilian Gonçalves Tort, Luis Fernando Lopez Pereira, Elisa Cavalcante Menezes, Lidiane Souza Raphael Malta, Fabio Correia de Oliveira, Barbara Cristina Euzebio Pereira Dias Rodrigues, João Paulo Manso, Pedro Paulo de Abreu Pelajo, Marcelo Bonaldo, Myrna Cristina Silva, Paola Cristina Resende Siqueira, Marilda Mendonça Brasil, Patricia Fumian, Tulio Machado Viruses Article The main objective of this study was to investigate the dynamic of SARS-CoV-2 viral excretion in rectal swab (RS), saliva, and nasopharyngeal swab (NS) samples from symptomatic patients and asymptomatic contacts. In addition, in order to evaluate the replication potential of SARS-CoV-2 in the gastrointestinal (GI) tract and the excretion of infectious SARS-CoV-2 from feces, we investigated the presence of subgenomic nucleoprotein gene (N) mRNA (sgN) in RS samples and cytopathic effects in Vero cell culture. A prospective cohort study was performed to collect samples from symptomatic patients and contacts in Rio de Janeiro, Brazil, from May to October 2020. One hundred and seventy-six patients had samples collected at home visits and/or during the follow up, resulting in a total of 1633 RS, saliva, or NS samples. SARS-CoV-2 RNA was detected in 130 (73.9%) patients who had at least one sample that tested positive for SARS-CoV-2. The presence of replicating SARS-CoV-2 in RS samples, measured by the detection of sgN mRNA, was successfully achieved in 19.4% (6/31) of samples, whilst infectious SARS-CoV-2, measured by the generation of cytopathic effects in cell culture, was identified in only one RS sample. Although rare, our results demonstrated the replication capacity of SARS-CoV-2 in the GI tract, and infectious viruses in one RS sample. There is still a gap in the knowledge regarding SARS-CoV-2 fecal–oral transmission. Additional studies are warranted to investigate fecal or wastewater exposure as a risk factor for transmission in human populations. MDPI 2023-05-11 /pmc/articles/PMC10224316/ /pubmed/37243238 http://dx.doi.org/10.3390/v15051152 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ribeiro, Ieda Pereira
do Nascimento, Lilian Gonçalves
Tort, Luis Fernando Lopez
Pereira, Elisa Cavalcante
Menezes, Lidiane Souza Raphael
Malta, Fabio Correia
de Oliveira, Barbara Cristina Euzebio Pereira Dias
Rodrigues, João Paulo
Manso, Pedro Paulo de Abreu
Pelajo, Marcelo
Bonaldo, Myrna Cristina
Silva, Paola Cristina Resende
Siqueira, Marilda Mendonça
Brasil, Patricia
Fumian, Tulio Machado
Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil
title Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil
title_full Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil
title_fullStr Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil
title_full_unstemmed Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil
title_short Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil
title_sort infectious sars-cov-2 particles from rectal swab samples from covid-19 patients in brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224316/
https://www.ncbi.nlm.nih.gov/pubmed/37243238
http://dx.doi.org/10.3390/v15051152
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