Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil
The main objective of this study was to investigate the dynamic of SARS-CoV-2 viral excretion in rectal swab (RS), saliva, and nasopharyngeal swab (NS) samples from symptomatic patients and asymptomatic contacts. In addition, in order to evaluate the replication potential of SARS-CoV-2 in the gastro...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224316/ https://www.ncbi.nlm.nih.gov/pubmed/37243238 http://dx.doi.org/10.3390/v15051152 |
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author | Ribeiro, Ieda Pereira do Nascimento, Lilian Gonçalves Tort, Luis Fernando Lopez Pereira, Elisa Cavalcante Menezes, Lidiane Souza Raphael Malta, Fabio Correia de Oliveira, Barbara Cristina Euzebio Pereira Dias Rodrigues, João Paulo Manso, Pedro Paulo de Abreu Pelajo, Marcelo Bonaldo, Myrna Cristina Silva, Paola Cristina Resende Siqueira, Marilda Mendonça Brasil, Patricia Fumian, Tulio Machado |
author_facet | Ribeiro, Ieda Pereira do Nascimento, Lilian Gonçalves Tort, Luis Fernando Lopez Pereira, Elisa Cavalcante Menezes, Lidiane Souza Raphael Malta, Fabio Correia de Oliveira, Barbara Cristina Euzebio Pereira Dias Rodrigues, João Paulo Manso, Pedro Paulo de Abreu Pelajo, Marcelo Bonaldo, Myrna Cristina Silva, Paola Cristina Resende Siqueira, Marilda Mendonça Brasil, Patricia Fumian, Tulio Machado |
author_sort | Ribeiro, Ieda Pereira |
collection | PubMed |
description | The main objective of this study was to investigate the dynamic of SARS-CoV-2 viral excretion in rectal swab (RS), saliva, and nasopharyngeal swab (NS) samples from symptomatic patients and asymptomatic contacts. In addition, in order to evaluate the replication potential of SARS-CoV-2 in the gastrointestinal (GI) tract and the excretion of infectious SARS-CoV-2 from feces, we investigated the presence of subgenomic nucleoprotein gene (N) mRNA (sgN) in RS samples and cytopathic effects in Vero cell culture. A prospective cohort study was performed to collect samples from symptomatic patients and contacts in Rio de Janeiro, Brazil, from May to October 2020. One hundred and seventy-six patients had samples collected at home visits and/or during the follow up, resulting in a total of 1633 RS, saliva, or NS samples. SARS-CoV-2 RNA was detected in 130 (73.9%) patients who had at least one sample that tested positive for SARS-CoV-2. The presence of replicating SARS-CoV-2 in RS samples, measured by the detection of sgN mRNA, was successfully achieved in 19.4% (6/31) of samples, whilst infectious SARS-CoV-2, measured by the generation of cytopathic effects in cell culture, was identified in only one RS sample. Although rare, our results demonstrated the replication capacity of SARS-CoV-2 in the GI tract, and infectious viruses in one RS sample. There is still a gap in the knowledge regarding SARS-CoV-2 fecal–oral transmission. Additional studies are warranted to investigate fecal or wastewater exposure as a risk factor for transmission in human populations. |
format | Online Article Text |
id | pubmed-10224316 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102243162023-05-28 Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil Ribeiro, Ieda Pereira do Nascimento, Lilian Gonçalves Tort, Luis Fernando Lopez Pereira, Elisa Cavalcante Menezes, Lidiane Souza Raphael Malta, Fabio Correia de Oliveira, Barbara Cristina Euzebio Pereira Dias Rodrigues, João Paulo Manso, Pedro Paulo de Abreu Pelajo, Marcelo Bonaldo, Myrna Cristina Silva, Paola Cristina Resende Siqueira, Marilda Mendonça Brasil, Patricia Fumian, Tulio Machado Viruses Article The main objective of this study was to investigate the dynamic of SARS-CoV-2 viral excretion in rectal swab (RS), saliva, and nasopharyngeal swab (NS) samples from symptomatic patients and asymptomatic contacts. In addition, in order to evaluate the replication potential of SARS-CoV-2 in the gastrointestinal (GI) tract and the excretion of infectious SARS-CoV-2 from feces, we investigated the presence of subgenomic nucleoprotein gene (N) mRNA (sgN) in RS samples and cytopathic effects in Vero cell culture. A prospective cohort study was performed to collect samples from symptomatic patients and contacts in Rio de Janeiro, Brazil, from May to October 2020. One hundred and seventy-six patients had samples collected at home visits and/or during the follow up, resulting in a total of 1633 RS, saliva, or NS samples. SARS-CoV-2 RNA was detected in 130 (73.9%) patients who had at least one sample that tested positive for SARS-CoV-2. The presence of replicating SARS-CoV-2 in RS samples, measured by the detection of sgN mRNA, was successfully achieved in 19.4% (6/31) of samples, whilst infectious SARS-CoV-2, measured by the generation of cytopathic effects in cell culture, was identified in only one RS sample. Although rare, our results demonstrated the replication capacity of SARS-CoV-2 in the GI tract, and infectious viruses in one RS sample. There is still a gap in the knowledge regarding SARS-CoV-2 fecal–oral transmission. Additional studies are warranted to investigate fecal or wastewater exposure as a risk factor for transmission in human populations. MDPI 2023-05-11 /pmc/articles/PMC10224316/ /pubmed/37243238 http://dx.doi.org/10.3390/v15051152 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ribeiro, Ieda Pereira do Nascimento, Lilian Gonçalves Tort, Luis Fernando Lopez Pereira, Elisa Cavalcante Menezes, Lidiane Souza Raphael Malta, Fabio Correia de Oliveira, Barbara Cristina Euzebio Pereira Dias Rodrigues, João Paulo Manso, Pedro Paulo de Abreu Pelajo, Marcelo Bonaldo, Myrna Cristina Silva, Paola Cristina Resende Siqueira, Marilda Mendonça Brasil, Patricia Fumian, Tulio Machado Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil |
title | Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil |
title_full | Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil |
title_fullStr | Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil |
title_full_unstemmed | Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil |
title_short | Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil |
title_sort | infectious sars-cov-2 particles from rectal swab samples from covid-19 patients in brazil |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10224316/ https://www.ncbi.nlm.nih.gov/pubmed/37243238 http://dx.doi.org/10.3390/v15051152 |
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