Protocol to isolate RBP-mRNA complexes using RNA-CLIP and examine target mRNAs

RNA-binding proteins (RBPs) regulate diverse functions by interacting with target transcripts. Here we present a protocol to isolate RBP-mRNA complexes using RNA-CLIP and examine target mRNAs in association with ribosomal populations. We describe steps to identify specific RBPs and RNA targets refle...

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Detalles Bibliográficos
Autores principales: Blanc, Valerie, Molitor, Elizabeth A., Davidson, Nicholas O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10225919/
https://www.ncbi.nlm.nih.gov/pubmed/37220002
http://dx.doi.org/10.1016/j.xpro.2023.102313

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10225919/
https://www.ncbi.nlm.nih.gov/pubmed/37220002
http://dx.doi.org/10.1016/j.xpro.2023.102313

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