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Protocol to isolate RBP-mRNA complexes using RNA-CLIP and examine target mRNAs
RNA-binding proteins (RBPs) regulate diverse functions by interacting with target transcripts. Here we present a protocol to isolate RBP-mRNA complexes using RNA-CLIP and examine target mRNAs in association with ribosomal populations. We describe steps to identify specific RBPs and RNA targets refle...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10225919/ https://www.ncbi.nlm.nih.gov/pubmed/37220002 http://dx.doi.org/10.1016/j.xpro.2023.102313 |