In vitro quantification of pigment production and transfer in 2D co-cultures and 3D skin organotypic

Here, we present a protocol to set up and study 2D keratinocyte-melanocyte co-cultures and 3D full-thickness human skin equivalents. We describe steps for culturing of keratinocyte and melanocyte lines and the establishment of both 2D and 3D co-cultures. The cultures are utilized to measure melanin...

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Detalles Bibliográficos
Autores principales: Phua, Rachel, Tien, Tracy Zhijun, Goh, Gracia, Pantelireis, Nikolaos, Sim, Kenice Siew Hoon, Lim, Jamien, Clavel, Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227445/
https://www.ncbi.nlm.nih.gov/pubmed/37243599
http://dx.doi.org/10.1016/j.xpro.2023.102334

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227445/
https://www.ncbi.nlm.nih.gov/pubmed/37243599
http://dx.doi.org/10.1016/j.xpro.2023.102334

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