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In vitro quantification of pigment production and transfer in 2D co-cultures and 3D skin organotypic
Here, we present a protocol to set up and study 2D keratinocyte-melanocyte co-cultures and 3D full-thickness human skin equivalents. We describe steps for culturing of keratinocyte and melanocyte lines and the establishment of both 2D and 3D co-cultures. The cultures are utilized to measure melanin...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227445/ https://www.ncbi.nlm.nih.gov/pubmed/37243599 http://dx.doi.org/10.1016/j.xpro.2023.102334 |