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Protocol for measuring transcytosis and recycling of IgG in intestinal epithelial Caco-2 cells and primary human intestinal organoids

Transcytosis is the primary mechanism by which macro-molecules transverse epithelial cell barriers. Here, we present an assay for measuring transcytosis and recycling of IgG in intestinal epithelial Caco-2 cells and primary human intestinal organoids. We describe steps for establishing human enteroi...

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Detalles Bibliográficos
Autores principales: Maeda, Keiko, Gwilt, Katlynn Bugda, Schmieder, Stefanie S., Zachos, Nicholas C., Lencer, Wayne I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227449/
https://www.ncbi.nlm.nih.gov/pubmed/37243601
http://dx.doi.org/10.1016/j.xpro.2023.102335