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Selection and validation of reference genes for RT-qPCR normalization of porcine alveolar macrophages (PAMs) for PRRSV studies

Porcine alveolar macrophages (PAMs) are widely used for in vitro studies of porcine respiratory viruses. Gene expression in these cells is altered by viral infection and cellular immune response. Real-time reverse transcription polymerase chain reaction (RT-qPCR) is a powerful technique for analyzin...

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Detalles Bibliográficos
Autores principales: Oh, Dayoung, De Spiegelaere, Ward, Nauwynck, Hans J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232543/
https://www.ncbi.nlm.nih.gov/pubmed/37258711
http://dx.doi.org/10.1038/s41598-023-35873-3