Site-specific gene knock-in and bacterial phytase gene expression in Chlamydomonas reinhardtii via Cas9 RNP-mediated HDR

In the present study, we applied the HDR (homology-directed DNA repair) CRISPR-Cas9-mediated knock-in system to accurately insert an optimized foreign bacterial phytase gene at a specific site of the nitrate reductase (NR) gene (exon 2) to achieve homologous recombination with the stability of the t...

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Detalles Bibliográficos
Autores principales: Zadabbas Shahabadi, Hassan, Akbarzadeh, Arash, Ofoghi, Hamideh, Kadkhodaei, Saeid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235511/
https://www.ncbi.nlm.nih.gov/pubmed/37275253
http://dx.doi.org/10.3389/fpls.2023.1150436

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235511/
https://www.ncbi.nlm.nih.gov/pubmed/37275253
http://dx.doi.org/10.3389/fpls.2023.1150436

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