Efficient precise integration of large DNA sequences with 3′-overhang dsDNA donors using CRISPR/Cas9

CRISPR/Cas9 genome-editing tools have tremendously boosted our capability of manipulating the eukaryotic genomes in biomedical research and innovative biotechnologies. However, the current approaches that allow precise integration of gene-sized large DNA fragments generally suffer from low efficienc...

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Detalles Bibliográficos
Autores principales: Han, Wenjie, Li, Zhigang, Guo, Yijun, He, Kaining, Li, Wenqing, Xu, Caoling, Ge, Lishuang, He, Miao, Yin, Xue, Zhou, Junxiang, Li, Chengxu, Yao, Dongbao, Bao, Jianqiang, Liang, Haojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2023
Materias:
Biological Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235934/
https://www.ncbi.nlm.nih.gov/pubmed/37216515
http://dx.doi.org/10.1073/pnas.2221127120

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235934/
https://www.ncbi.nlm.nih.gov/pubmed/37216515
http://dx.doi.org/10.1073/pnas.2221127120

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