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Efficient precise integration of large DNA sequences with 3′-overhang dsDNA donors using CRISPR/Cas9
CRISPR/Cas9 genome-editing tools have tremendously boosted our capability of manipulating the eukaryotic genomes in biomedical research and innovative biotechnologies. However, the current approaches that allow precise integration of gene-sized large DNA fragments generally suffer from low efficienc...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
National Academy of Sciences
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235934/ https://www.ncbi.nlm.nih.gov/pubmed/37216515 http://dx.doi.org/10.1073/pnas.2221127120 |