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A method for parallel microscale protein labeling and precise control over the average degree of labeling (aDoL)

A widely used approach for protein conjugation is through the lysine residues reacting with NHS- or other active esters. However, it is a challenge to precisely control the degree of labeling (DoL) due to the instability of active ester and variability of reaction efficiencies. Here, we provide a pr...

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Detalles Bibliográficos
Autores principales: Ruan, Qiaoqiao, Zhao, Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10238424/
https://www.ncbi.nlm.nih.gov/pubmed/37268718
http://dx.doi.org/10.1038/s41598-023-36163-8