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Protocol for analyzing intact mRNA poly(A) tail length using nanopore direct RNA sequencing
Poly(A) tail metabolism contributes to post-transcriptional regulation of gene expression. Here, we present a protocol for analyzing intact mRNA poly(A) tail length using nanopore direct RNA sequencing, which excludes truncated RNAs from the measurement. We describe steps for preparing recombinant e...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10239010/ https://www.ncbi.nlm.nih.gov/pubmed/37243600 http://dx.doi.org/10.1016/j.xpro.2023.102340 |