Protocol for analyzing intact mRNA poly(A) tail length using nanopore direct RNA sequencing

Poly(A) tail metabolism contributes to post-transcriptional regulation of gene expression. Here, we present a protocol for analyzing intact mRNA poly(A) tail length using nanopore direct RNA sequencing, which excludes truncated RNAs from the measurement. We describe steps for preparing recombinant e...

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Detalles Bibliográficos
Autores principales: Ogami, Koichi, Oishi, Yuka, Hoshino, Shin-ichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10239010/
https://www.ncbi.nlm.nih.gov/pubmed/37243600
http://dx.doi.org/10.1016/j.xpro.2023.102340

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10239010/
https://www.ncbi.nlm.nih.gov/pubmed/37243600
http://dx.doi.org/10.1016/j.xpro.2023.102340

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