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Pregranulosa cell–derived FGF23 protects oocytes from premature apoptosis during primordial follicle formation by inhibiting p38 MAPK in mice

A large number of oocytes in the perinatal ovary in rodents get lost for unknown reasons. The granulosa cell–oocyte mutual communication is pivotal for directing formation of the primordial follicle; however, little is known if paracrine factors participate in modulating programmed oocyte death peri...

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Autores principales: Zhu, Zijian, Qin, Shaogang, Zhang, Tuo, He, Meina, Zheng, Wenying, Zhao, Ting, Gao, Meng, Chen, Ziqi, Zhou, Bo, Xia, Guoliang, Wang, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10240423/
https://www.ncbi.nlm.nih.gov/pubmed/37142227
http://dx.doi.org/10.1016/j.jbc.2023.104776
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author Zhu, Zijian
Qin, Shaogang
Zhang, Tuo
He, Meina
Zheng, Wenying
Zhao, Ting
Gao, Meng
Chen, Ziqi
Zhou, Bo
Xia, Guoliang
Wang, Chao
author_facet Zhu, Zijian
Qin, Shaogang
Zhang, Tuo
He, Meina
Zheng, Wenying
Zhao, Ting
Gao, Meng
Chen, Ziqi
Zhou, Bo
Xia, Guoliang
Wang, Chao
author_sort Zhu, Zijian
collection PubMed
description A large number of oocytes in the perinatal ovary in rodents get lost for unknown reasons. The granulosa cell–oocyte mutual communication is pivotal for directing formation of the primordial follicle; however, little is known if paracrine factors participate in modulating programmed oocyte death perinatally. We report here that pregranulosa cell–derived fibroblast growth factor 23 (FGF23) functioned in preventing oocyte apoptosis in the perinatal mouse ovary. Our results showed that FGF23 was exclusively expressed in pregranulosa cells, while fibroblast growth factor receptors (FGFRs) were specifically expressed in the oocytes in perinatal ovaries. FGFR1 was one of the representative receptors in mediating FGF23 signaling during the formation of the primordial follicle. In cultured ovaries, the number of live oocytes declines significantly, accompanied by the activation of the p38 mitogen-activated protein kinase signaling pathway, under the condition of FGFR1 disruption by specific inhibitors of FGFR1 or silencing of Fgf23. As a result, oocyte apoptosis increased and eventually led to a decrease in the number of germ cells in perinatal ovaries following the treatments. In the perinatal mouse ovary, pregranulosa cell–derived FGF23 binds to FGFR1 and activates at least the p38 mitogen-activated protein kinase signaling pathway, thereby regulating the level of apoptosis during primordial follicle formation. This study reemphasizes the importance of granulosa cell–oocyte mutual communication in modulating primordial follicle formation and supporting oocyte survival under physiological conditions.
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spelling pubmed-102404232023-06-06 Pregranulosa cell–derived FGF23 protects oocytes from premature apoptosis during primordial follicle formation by inhibiting p38 MAPK in mice Zhu, Zijian Qin, Shaogang Zhang, Tuo He, Meina Zheng, Wenying Zhao, Ting Gao, Meng Chen, Ziqi Zhou, Bo Xia, Guoliang Wang, Chao J Biol Chem Research Article A large number of oocytes in the perinatal ovary in rodents get lost for unknown reasons. The granulosa cell–oocyte mutual communication is pivotal for directing formation of the primordial follicle; however, little is known if paracrine factors participate in modulating programmed oocyte death perinatally. We report here that pregranulosa cell–derived fibroblast growth factor 23 (FGF23) functioned in preventing oocyte apoptosis in the perinatal mouse ovary. Our results showed that FGF23 was exclusively expressed in pregranulosa cells, while fibroblast growth factor receptors (FGFRs) were specifically expressed in the oocytes in perinatal ovaries. FGFR1 was one of the representative receptors in mediating FGF23 signaling during the formation of the primordial follicle. In cultured ovaries, the number of live oocytes declines significantly, accompanied by the activation of the p38 mitogen-activated protein kinase signaling pathway, under the condition of FGFR1 disruption by specific inhibitors of FGFR1 or silencing of Fgf23. As a result, oocyte apoptosis increased and eventually led to a decrease in the number of germ cells in perinatal ovaries following the treatments. In the perinatal mouse ovary, pregranulosa cell–derived FGF23 binds to FGFR1 and activates at least the p38 mitogen-activated protein kinase signaling pathway, thereby regulating the level of apoptosis during primordial follicle formation. This study reemphasizes the importance of granulosa cell–oocyte mutual communication in modulating primordial follicle formation and supporting oocyte survival under physiological conditions. American Society for Biochemistry and Molecular Biology 2023-05-02 /pmc/articles/PMC10240423/ /pubmed/37142227 http://dx.doi.org/10.1016/j.jbc.2023.104776 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Zhu, Zijian
Qin, Shaogang
Zhang, Tuo
He, Meina
Zheng, Wenying
Zhao, Ting
Gao, Meng
Chen, Ziqi
Zhou, Bo
Xia, Guoliang
Wang, Chao
Pregranulosa cell–derived FGF23 protects oocytes from premature apoptosis during primordial follicle formation by inhibiting p38 MAPK in mice
title Pregranulosa cell–derived FGF23 protects oocytes from premature apoptosis during primordial follicle formation by inhibiting p38 MAPK in mice
title_full Pregranulosa cell–derived FGF23 protects oocytes from premature apoptosis during primordial follicle formation by inhibiting p38 MAPK in mice
title_fullStr Pregranulosa cell–derived FGF23 protects oocytes from premature apoptosis during primordial follicle formation by inhibiting p38 MAPK in mice
title_full_unstemmed Pregranulosa cell–derived FGF23 protects oocytes from premature apoptosis during primordial follicle formation by inhibiting p38 MAPK in mice
title_short Pregranulosa cell–derived FGF23 protects oocytes from premature apoptosis during primordial follicle formation by inhibiting p38 MAPK in mice
title_sort pregranulosa cell–derived fgf23 protects oocytes from premature apoptosis during primordial follicle formation by inhibiting p38 mapk in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10240423/
https://www.ncbi.nlm.nih.gov/pubmed/37142227
http://dx.doi.org/10.1016/j.jbc.2023.104776
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