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Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platform
Reverse genetic systems enable the engineering of RNA virus genomes and are instrumental in studying RNA virus biology. With the recent outbreak of the COVID-19 pandemic, already established methods were challenged by the large genome of SARS-CoV-2. Herein we present an elaborated strategy for the r...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10245781/ https://www.ncbi.nlm.nih.gov/pubmed/37292682 http://dx.doi.org/10.1101/2023.05.11.540343 |