Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platform

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Reverse genetic systems enable the engineering of RNA virus genomes and are instrumental in studying RNA virus biology. With the recent outbreak of the COVID-19 pandemic, already established methods were challenged by the large genome of SARS-CoV-2. Herein we present an elaborated strategy for the r...

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Detalles Bibliográficos
Autores principales: Kipfer, Enja, Hauser, David, Lett, Martin J., Otte, Fabian, Urda, Lorena, Zhang, Yuepeng, Lang, Christopher M. R., Chami, Mohamed, Mittelholzer, Christian, Klimkait, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10245781/
https://www.ncbi.nlm.nih.gov/pubmed/37292682
http://dx.doi.org/10.1101/2023.05.11.540343
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