In vitro reconstitution of SARS CoV-2 Nsp1-induced mRNA cleavage reveals the key roles of the N-terminal domain of Nsp1 and the RRM domain of eIF3g

SARS CoV-2 nonstructural protein 1 (Nsp1) is the major pathogenesis factor that inhibits host translation using a dual strategy of impairing initiation and inducing endonucleolytic cleavage of cellular mRNAs. To investigate the mechanism of cleavage, we reconstituted it in vitro on β-globin, EMCV IR...

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Detalles Bibliográficos
Autores principales: Abaeva, Irina S., Arhab, Yani, Miścicka, Anna, Hellen, Christopher U. T., Pestova, Tatyana V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10245999/
https://www.ncbi.nlm.nih.gov/pubmed/37292671
http://dx.doi.org/10.1101/2023.05.25.542379

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10245999/
https://www.ncbi.nlm.nih.gov/pubmed/37292671
http://dx.doi.org/10.1101/2023.05.25.542379

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