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Risk assessment and bioburden evaluation of Agrobacterium tumefaciens-mediated transient protein expression in plants using the CaMV35S promoter

Large-scale transient expression of recombinant proteins in plants is increasingly used and requires the multi-liter cultivation of Agrobacterium tumefaciens transformed with an expression vector, which is often cloned in Escherichia coli first. Depending on the promoter, unintentional activity can...

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Detalles Bibliográficos
Autores principales: Knödler, Matthias, Reunious, Paul Winman, Buyel, Johannes Felix
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246419/
https://www.ncbi.nlm.nih.gov/pubmed/37286972
http://dx.doi.org/10.1186/s12896-023-00782-w