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Impact of antigen retrieval protocols on the immunohistochemical detection of epigenetic DNA modifications

This study compares three different pretreatment protocols for the immunohistochemical detection of 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC) in nuclear DNA. The human biological samples analyzed included formalin-fixed and paraffin-embedded (FFPE) normal squamous epithelium, ethan...

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Detalles Bibliográficos
Autores principales: Moshi, Jobran M., Ummelen, Monique, Broers, Jos L. V., Ramaekers, Frans C. S., Hopman, Anton H. N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247850/
https://www.ncbi.nlm.nih.gov/pubmed/37010548
http://dx.doi.org/10.1007/s00418-023-02187-4