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Optimization and evaluation of viral metagenomic amplification and sequencing procedures toward a genome-level resolution of the human fecal DNA virome

INTRODUCTION: Viruses in the human gut have been linked to health and disease. Deciphering the gut virome is dependent on metagenomic sequencing of the virus-like particles (VLPs) purified from the fecal specimens. A major limitation of conventional viral metagenomic sequencing is the low recoverabi...

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Detalles Bibliográficos
Autores principales: Wang, Guangyang, Li, Shenghui, Yan, Qiulong, Guo, Ruochun, Zhang, Yue, Chen, Fang, Tian, Xiangge, Lv, Qingbo, Jin, Hao, Ma, Xiaochi, Ma, Yufang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10248800/
https://www.ncbi.nlm.nih.gov/pubmed/35995413
http://dx.doi.org/10.1016/j.jare.2022.08.011