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Temperature-Dependent Affinity Changes in Substrate Binding Affect the Cleavage Activity of BthC2c1

BACKGROUND: The CRISPR-Cas system is an adaptive immune mechanism for bacteria and archaea to resist foreign invasion. Currently, Cas9 and Cpf1 have been widely studied and applied in gene editing. C2c1 is a newly discovered CRISPR-Cas system endonuclease. It has broad application prospects due to i...

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Detalles Bibliográficos
Autores principales:	Wu, Dan, Liu, Jieting, Liu, Yong, Qiu, Yufei, Cao, Zhiqin, Pan, Yu, Shi, Jiayi, Yuan, Xiaohuan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Bentham Science Publishers 2023
Materias:	Life Sciences, Protein and Peptide Sciences, Biochemistry and Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10249141/ https://www.ncbi.nlm.nih.gov/pubmed/36698226 http://dx.doi.org/10.2174/0929866530666230125100320

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