A dual gene-specific mutator system installs all transition mutations at similar frequencies in vivo

Targeted in vivo hypermutation accelerates directed evolution of proteins through concurrent DNA diversification and selection. Although systems employing a fusion protein of a nucleobase deaminase and T7 RNA polymerase present gene-specific targeting, their mutational spectra have been limited to e...

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Detalles Bibliográficos
Autores principales: Seo, Daeje, Koh, Bonghyun, Eom, Ga-eul, Kim, Hye Won, Kim, Seokhee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10250238/
https://www.ncbi.nlm.nih.gov/pubmed/37070179
http://dx.doi.org/10.1093/nar/gkad266

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10250238/
https://www.ncbi.nlm.nih.gov/pubmed/37070179
http://dx.doi.org/10.1093/nar/gkad266

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